Extraction of Paclitaxel by rapid chromatography method

Paclitaxel is the most popular anti-cancer drug on international market in recent years. It is considered to be one of the most effective anticancer drugs in the next 20 years. In recent years, with the explosive growth of population and cancer incidence, the demand for Paclitaxel has also increased significantly. Paclitaxel is mainly extracted from Taxus chinensis trees, but this method is limited by the number of Taxus chinensis trees. The Paclitaxel on the market is main get by that scientists use rapid liquid chromatography to separate and purify Taxus chinensis plant extracts.

Experiment Part

The sample is a Paclitaxel plant extract liquid, then dissolving in DMSO, at last sampled into chromatography instrument system.

Instrument	Liquid instrument
Chromatography column	12 g
Test the wavelength	254 nm; 280nm
Mobile phase	Solvent A: Water    Solvent B: methyl alcohol
Flow rate	15ml/min
Injection volume	20 mg crude plant extract dissolved in 1ml DMSO
Elution gradient	Time (min)	Solvent B (%)
	0	10
	5	10
	7	28
	14	28
	16	40

Conclusion

Analysis the chromatography data, it showed that the target product and impurities have been separated from the baseline, and repeated multiple some times, the chromatogram is accorded. Compared with the traditional manual 4h cross-bar ingress, the automation method requires 44min, which can raise efficiency more than 80% , and save a large amount of elution solvent. In this method, 2ml screw vial should be used. Aijiren is a chromatography consumables supplier, which is screw chromatography vial, snap vial and crimp vial, closures and caps supplier for sale. Please inquiry us for newest price list.